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National Academy of Medical Sciences (India) |
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CME Monograph IV (2001) “Mycobacteriology Laboratory Manual”, Editors: Drs. P. Venkataraman*, C.N. Paramasivan* and J.N. Pande@, *Tuberculosis Research Center (ICMR), Chetput, Chennai, @All India Institute of Medical Sciences, New Delhi
Contents
Sputum Smear Microscopy Equipment & reagents Specimen collection Smear preparation procedures Acid-Fast staining procedures Ziehl-Neelsen staining Smear examination procedures Morphological characteristics of tubercle bacilli Causes of error in microscopy Consequences of false-positive and false-negative smears Recording and reporting of results Essential equipment and supplies References
Culture of Sputum Specimens Specimen collection Homogenization and Decontamination Modified Petroff’s method Processing of specimens with CPC and NaCl Culture media Advantages and disadvantages of Egg-based medium Precautions during media preparation Preparation of Lowenstein-Jensen medium Inoculation and Incubation procedures Culture examination and identification Reading of cultures Recording and reporting of laboratory results Care and maintenance of essential equipment Safe handling of specimens Essential equipment and supplies References
Drug Susceptibility Tests Bacterial suspension Incubation and reading Interpretation of results Definition of resistance Preparation of drug-containing media References
Identification Tests Niacin test Catalase test Growth on PNB medium Summary References
Quality Assurance Programme Quality control Quality improvement Proficiency testing References
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2005 National Academy of Medical Sciences (India) All Rights Reserved |