National Academy of Medical Sciences (India)








CME Monograph V (2003)

“Mollicutes Technology and Molecular Techniques”, Editors: Drs. Rama Chaudhry, Pawan Malhotra and Usha Gupta, All India Institute of Medical Sciences, New Delhi



Title of Topic                                                              Author(s)



Mycoplasma in Human Infections                            Meera Sharma, Sunil Sethi and
                                                                                    Caesar Sengupta


Isolation, Cultivation and Maintenance                   Nazima Nisar and Rama Chaudhry
of Mycoplasma pneumoniae
Diagnosis and Antimicrobial Susceptibility


Laboratory Diagnosis and Antimicrobial                  Ken B. Waites
Susceptibility Testing of Mycoplasmal and
Ureaplasmal Infections in Humans


Serodiagnosis of Mycoplasma pneumoniae by        Anita Pandey, Nazima Nisar, Benu


Enzyme Linked Immunosorbent Assay (ELISA)     Dhawan and Rama Chaudhry
and Gelatin Particle Agglutination (GPA) test


Indirect Immunofluorescence test for detection      Rama Chaudhry, Nazima Nisar
of Mycoplasma pneumoniae in clinical samples      Anita Pandey


Analysis of Mycoplasma pneumoniae Proteins       Nazima Nisar, Rama Chaudhry
By SDS-Polyacrylamide Gel Electrophoresis


Immunoblotting                                                          Vikas K. Goel, Arti Kapil


Methods for Detection of Mycoplasma                   Immaculata Xess, Rama Chaudhry
Contamination in Tissue Culture
Molecular Techniques


Introduction to Molecular Biology                            Shobha Broor


Extraction and Preparation of DNA                         Lalit Dar
from Bacteria


PCR and its Applications                                           Dinesh S Chandel, Nazima Nisar,
                                                                                     Rama Chaudhry


 Agarose Gel Electrophoresis                                    Sukhbir K. Chadha, Nazima Nisar


 Cloning and Expression of PCR Products                Alka Arora


 DNA Probing                                                            Dinesh S. Chandel, Himanshu P. Raikwar


Restriction Fragment Length Polymorphism           Dinesh S. Chandel, Lt. Col. A.K. Sahni


DNA Sequencing Methods                                       Pawan Malhotra


DNA Sequencing by Sanger’s Dideoxy                    Arindam Maitra
Chain Termination Method


Automated DNA Sequencing                                    Urvashi B. Singh


Pulse Field Gel Electrophoresis                                Madhu Vajpayee


Biosafety Guidelines                                                 Nidhi Sharma, Purva Mathur




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