CME Monograph V (2003)

“Mollicutes Technology and Molecular Techniques”, Editors: Drs. Rama Chaudhry, Pawan Malhotra and Usha Gupta, All India Institute of Medical Sciences, New Delhi

Contents:

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Title of Topic                                                              Author(s)

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General

Mycoplasma in Human Infections                            Meera Sharma, Sunil Sethi and
                                                                                    Caesar Sengupta

Culture

Isolation, Cultivation and Maintenance                   Nazima Nisar and Rama Chaudhry
of Mycoplasma pneumoniae
Diagnosis and Antimicrobial Susceptibility

Laboratory Diagnosis and Antimicrobial                  Ken B. Waites
Susceptibility Testing of Mycoplasmal and
Ureaplasmal Infections in Humans

Serology
Serodiagnosis of Mycoplasma pneumoniae by        Anita Pandey, Nazima Nisar, Benu

Enzyme Linked Immunosorbent Assay (ELISA)     Dhawan and Rama Chaudhry
and Gelatin Particle Agglutination (GPA) test

Indirect Immunofluorescence test for detection      Rama Chaudhry, Nazima Nisar
of Mycoplasma pneumoniae in clinical samples      Anita Pandey

Analysis of Mycoplasma pneumoniae Proteins       Nazima Nisar, Rama Chaudhry
By SDS-Polyacrylamide Gel Electrophoresis

Immunoblotting                                                          Vikas K. Goel, Arti Kapil

Methods for Detection of Mycoplasma                   Immaculata Xess, Rama Chaudhry
Contamination in Tissue Culture
Molecular Techniques

Introduction to Molecular Biology                            Shobha Broor

Extraction and Preparation of DNA                         Lalit Dar
from Bacteria

PCR and its Applications                                           Dinesh S Chandel, Nazima Nisar,
                                                                                     Rama Chaudhry

 Agarose Gel Electrophoresis                                    Sukhbir K. Chadha, Nazima Nisar

 Cloning and Expression of PCR Products                Alka Arora

 DNA Probing                                                            Dinesh S. Chandel, Himanshu P. Raikwar

Restriction Fragment Length Polymorphism           Dinesh S. Chandel, Lt. Col. A.K. Sahni

DNA Sequencing Methods                                       Pawan Malhotra

DNA Sequencing by Sanger’s Dideoxy                    Arindam Maitra
Chain Termination Method

Automated DNA Sequencing                                    Urvashi B. Singh

Pulse Field Gel Electrophoresis                                Madhu Vajpayee

Biosafety Guidelines                                                 Nidhi Sharma, Purva Mathur

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