CME Monograph IV (2001)

“Mycobacteriology Laboratory Manual”
  Editors: Drs. P. Venkataraman*, C.N. Paramasivan* and J.N. Pande@, *Tuberculosis Research Center (ICMR), Chetput, Chennai, @All India Institute of Medical Sciences, New Delhi

Sputum Smear Microscopy

Equipment & reagents
Specimen collection
Smear preparation procedures
Acid-Fast staining procedures
Ziehl-Neelsen staining
Smear examination procedures
Morphological characteristics of tubercle bacilli
Causes of error in microscopy
Consequences of false-positive and false-negative smears
Recording and reporting of results
Essential equipment and supplies
References

Culture of Sputum Specimens

Specimen collection
Homogenization and Decontamination
Modified Petroff’s method
Processing of specimens with CPC and NaCl
Culture media
Advantages and disadvantages of Egg-based medium
Precautions during media preparation
Preparation of Lowenstein-Jensen medium
Inoculation and Incubation procedures
Culture examination and identification
Reading of cultures
Recording and reporting of laboratory results
Care and maintenance of essential equipment
Safe handling of specimens
Essential equipment and supplies
References

Drug Susceptibility Tests

Bacterial suspension
Incubation and reading
Interpretation of results
Definition of resistance
Preparation of drug-containing media
References

Identification Tests

Niacin test
Catalase test
Growth on PNB medium
Summary
References

Quality Assurance Programme

Quality control
Quality improvement
Proficiency testing
References